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Unformatted text preview: lues for the amino acids, draw the expected structure of the chymotrypsin catalytic
triad at pH 7.5 and at pH 5.5, including interactions between the side chains. Based on
this analysis, suggest why the catalytic activity might be lower at pH 5.5 than at pH 7.5.
Based on a typical His side chain pKa of 6-7, at pH 5.5 the His of the triad will be
protonated and will not be poised to deprotonate the serine to generate the nucleophile.
H O N H N pH 7.5
O H N N H pH 5.5
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This test prep was uploaded on 02/25/2014 for the course BIOC 462A taught by Professor Ziegler,baldwin during the Spring '08 term at University of Arizona- Tucson.
- Spring '08