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2. As discussed in lecture, DIPF is a group-specific reagent that inactivates chymotrypsin.
Would you expect it to inactivate subtilisin? Papain? HIV protease? Explain why or why
not in each case, basing your answer on what you learned in lecture about the active sites
of these enzymes.
Yes to subtilisin, no to papain or HIV protease. Subtilisin is a serine protease, papain is a
cysteine protease and HIV protease is an aspartyl protease. Only subtilisin has a serine in
its active site that DIPF would be expected to react with.
3. Chymotrypsin has a lower kcat at pH 5.5 than at pH 7.5. Based on standard side-chain
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This test prep was uploaded on 02/25/2014 for the course BIOC 462A taught by Professor Ziegler,baldwin during the Spring '08 term at University of Arizona- Tucson.
- Spring '08