You do michaelis menten kinetics and find that km for

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: determine the amino-acid sequence of clypsin A. It is found to be homologous to trypsin and chymotrypsin. From sequence alignments, you can identify the key specificity pocket residues as Thr 189-Ala 216-Val 226. You later discover that the monkey has an isozyme of this serine protease, clypsin B, in which residue 189 is Ser instead of Thr. You do Michaelis-Menten kinetics and find that KM for clypsin B with FAVE is 2 times higher than KM for clypsin A with FAVE, while KM for clypsin B with FALE is 2 times lower than KM for clypsin A with FALE. Propose an explanation for these findings. The Thr to Ser replacement probably increases the size...
View Full Document

Ask a homework question - tutors are online