Q which of the following statements about

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Unformatted text preview: •  Kd = the concentra=on of ligand needed to HALF ­SATURATE the binding sites. Plot analysis of ligand binding experiments [PL ] [L ] "= = [PL ] + [P] K d + [L ] #1& 1 = Kd% ( + 1 " $ [L ] ' slope = Kd 1/θ o o o ooo oo 1/[L] •  Data points tend to gather on one side Possibly induces large error •  Current approach: use nonlinear fi]ng program to the bare data 2 proteins binding the same ligand Which one has the higher binding affinity for the ligand? Q. Which of the following statements about protein ­ligand binding is correct? A.  The Ka is equal to the concentra=on of ligand when all of the binding sites are occupied. B.  The Ka is independent of such condi=ons as salt concentra=on and pH. C.  The larger the Ka (associa=on constant), the weaker the affinity. D.  The larger the Ka, the faster is the binding. E.  The larger the Ka, the smaller the Kd (dissocia=on constant). MYOGLOBIN AND HEMOGLOBIN: PARADIGMS OF LIGAND BINDING, PROTEIN FUNCTION AND REGULATION •  Oxygen –  Required for oxidative metabolism and energy production (most cells) –  O2 = terminal electron acceptor in aerobic metabolism (dietary substrates oxidized to CO2, yielding energy) •  Hemoglobin (Hb) function (vertebrate erythrocytes) –  transport O2 from lungs to tissues (or gills), AND –  transport CO2 from tissues to lungs (or gills) •  Myoglobin (Mb) function (esp. muscle tissue) –  bind O2 delivered to tissue by blood –  store O2 until needed as terminal e– acceptor for energ...
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This test prep was uploaded on 02/25/2014 for the course BIOC 462A taught by Professor Ziegler,baldwin during the Spring '08 term at University of Arizona- Tucson.

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