L34_nucleicacids2_462a_2013

Why least stacking energy ie low stability stacked

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Unformatted text preview: omaMc rings essenMally parallel to ring systems above and below) by van der Waals and dipole ­dipole interacMons (enthalpic factor). Stacking energies for 10 possible dimers in B ­DNA most stacking energy (i.e. high stability) Rule of thumb: InteracMons between stacked G and C bases are greater than those between stacked A and T bases DNAs with a high G + C content are generally more stable. Hydrogen bonds between base pairs are important in specificity of strand interacMons, (much less effect on stability of double ­ stranded structure). Why? least stacking energy (i.e. low stability) Stacked dimer Stacking energy (kJ/ mol) C•G G•C  ­ 61.0 C•G A•T  ­ 44.0 C•G T•A  ­ 41.0 G•C C•G  ­ 40.5 G•C G•C  ­ 34.6 G•C A•T  ­ 28.4 T•A A•T  ­ 27.5 G•C T•A  ­ 27.5 A•T A•T  ­ 22.5 A•T T•A  ­ 16.0 Monitoring denaturaMon and renaturaMon of nucleic acids Bases absorb...
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This test prep was uploaded on 02/25/2014 for the course BIOC 462A taught by Professor Ziegler,baldwin during the Spring '08 term at University of Arizona- Tucson.

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