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Unformatted text preview: Green white --> yellow pink a. What colour is the prototroph strain? (1) White b. Choose one step of the pathway to be mutated (cross out the appropriate arrow). What colour is the auxotroph created by this mutation? (1) Auxotroph will be the colour before the arrow you crossed out, e.g. if you crossed out the second arrow, it will be yellow. c. In a syntrophic feeding experiment, which strain(s) (if any) would you need to feed your mutant to make the end product? (1) Any colour after your auxotroph, except for pink, since this is the prototroph and doesnt make excess product. d. You are given a brown auxotroph that feeds mutually with the yellow strain. Add the appropriate extra step to the pathway to include the brown intermediate. (1) Branched pathway: Green brown white (parallel to branch with yellow)...
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- Spring '06