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chapter_14 - Some terminology genetic engineering Chapter...

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1 1 Chapter 14 Recombinant DNA Technology 2 Some terminology genetic engineering deliberate modification of organism’s genetic information by directly changing its genome recombinant DNA technology procedures used to carry out genetic engineering biotechnology use of organisms to form useful products 3 Historical Perspectives 4 Restriction enzymes cleave DNA at specific sequences first discovered by Arber and Smith (late 1960s) Boyer (1969) first isolated Eco RI
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2 5 Figure 14.2 sticky ends 6 Reverse transcriptase synthesizes double stranded DNA from RNA template used to construct complementary DNA (cDNA) independently discovered by Temin and Baltimore in 1970 7 The Polymerase Chain Reaction synthesizes large quantity of a DNA fragment without cloning it three steps repeated denature target DNA with heat anneal to primers specific for target DNA synthesize copies of target DNA 8 often a synthetic oligonucleotide usually catalyzed by
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