F13 lecture 6 for post

Zoologysiuedu heistimages sunfishallozymejpg taq

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Unformatted text preview: sed on amino acid sequences) BIO 367/BEE 567 lecture 1 20 Allozymes •  =“allele” + “enzyme” •  Polymorphisms involving charge differences in metabolic enzymes •  Recognized using electrophore1c separa1on of protein extracts •  Lewon1n and Hubby (1966) –  Examined 18 gene loci from 6 popula1ons (Drosophila pseudoobscura) –  Only 11 of 18 loci were monomorphic (no allozyme varia1on) –  39% of loci turned out to be polymorphic for allozymes –  Led to predic1on: 8- 15% of all loci will be polymorphic (typically expressed as likelihood an individual will be BIO367/BEE 567 Lab 1 Fall 05 heterozygous) http://en2.wikipedia.org/wiki/Amino_acid BIO367/BEE 567 Lab 1 Fall 05 =usually negative at physiological pH =usually positive at physiological pH =sometimes positive at physiological pH Amino acid subs1tu1ons •  May or may not change the charge of the protein •  If net charge of protein is altered, electrophore1c mobility will change –  Basis of “allozymes” (allele + enzyme) BIO367/BEE 567 Lab 1 Fall 05 BIO367/BEE 567 Lab 1 Fall 05 Allozymes are co-dominant markers =can tell all three genotypes previous...
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This document was uploaded on 02/27/2014.

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