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F13 lecture 4

# Interval recombination between b and c 111554323 108

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Unformatted text preview: DUALS - 125 - 114 0 ry + + ------ry e ro 1 1ST + e ro ------ry e ro 1 1ST ry e + ------ry e ro RECOMBINANTS 0 +++ ------ry e ro PARENTALS ry e ro ------ry e ro 1 2ND 28 + + ro ------ry e ro 1 2ND 31 ry + ro ------ry e ro 2 1ST and 2ND 4 +e+ ------ry e ro 2 1ST and 2ND 8 TOTAL= 23 29 362 # RECOMBINATIONS RECOMB. INTERVAL recombination between ry and e (1st interval) = (23+29+4+8)/362 = 17.7% # INDIVIDUALS ry e ro ------ry e ro 0 - 125 +++ ------ry e ro 0 - 114 ry + + ------ry e ro 1 1ST 23 + e ro ------ry e ro 1 1ST 29 ry e + ------ry e ro 1 2ND 28 + + ro ------ry e ro 1 2ND 31 ry + ro ------ry e ro 2 1ST and 2ND 4 +e+ ------ry e ro 2 1ST and 2ND 8 TOTAL= 362 # RECOMBINATIONS RECOMB. INTERVAL recombination between e and ro (2nd interval) = (28+31+4+2)/362 = 19.6% # INDIVIDUALS ry e ro ------ry e ro 0 - 125 +++ ------ry e ro 0 - 114 ry + + ------ry e ro 1 1ST 23 + e ro ------ry e ro 1 1ST 29 ry e + ------ry e ro 1 2ND 28 + + ro ------ry e ro 1 2ND 31 ry + ro ------ry e ro 2 1ST and 2ND 4 +e+ ------ry e ro 2 1ST and 2ND 8 TOTAL= 362 rosy (ry) ebony (e) rough (ro) | | | 17.7 19.6 We could also treat ry-e as an interval and measure the overall recomb rate in this interval -in practice this is less accurate than determining the small intervals and summing them previous example •  map order known •  map distances known (D. melanogaster) •  what about data with three linked markers of unknown order? –  three possible orders: •  abc •  acb •  cab –  how do we dete...
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