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When and why some haloarchaea need bacteriorhodopsin

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Unformatted text preview: n pumps Bacteriorhodopsin Halorhodopsin Discovered in 1973 Discovered in 1980 A single- site mutaCon in bacteriorhodopsin converts it from a proton pump to a Cl– pump. Share sequence idenCCes (25–35%) with bacteriorhodopsins ReCnal- containing protein Integral membrane proteins with seven alpha helices across the cytoplasmic membrane, surrounding the reCnal- binding pocket Light- driven proton pump (outward) 11 Light- driven anion pump (inward) 12 2 14/04/13 How does Bacteriorhodopsin work? When and why some haloarchaea need Bacteriorhodopsin? In Bacteriorhodopsin, a chromophore, reCnal, is covalently linked, as a protonated Schiff base, to a lysine residue in the seventh transmembrane helix. ­༊ Most of haloarchaea are obligate aerobes that is they depend on aerobic respiraCon. ­༊ As living environment (i.e. saltern) approach the minimum...
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