Chapter 18 Study Questions--answers at end

Chapter 18 Study Questions--answers at end - CHAPTER 18...

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Unformatted text preview: CHAPTER 18 STUDY QUESTIONS 1) Eukaryotes utilize at least one method to control their gene expression that is not seen in bacteria. This method (or methods) is (are): A. control of chromatin remodeling B. control of RNA processing, especially splicing C. transcriptional control D. answers A and B E. all of the above 2) Which of the following properties do you predict to be most critical to histone association with DNA? A. Histones are small proteins. B. Histones are highly conserved (i.e., histones are very similar in every eukaryote). C. Histones are synthesized in the cytoplasm. D. There are at least five different histone proteins in every eukaryote. E. Histones are positively charged. 3) The following figure is an electron micrograph showing the so-called beads-on-a-string structure of chromatin. If this preparation of chromatin formed the next higher level of chromatin structure, what would be seen is: A. DNA without protein B. the 30-nanometer fiber C. a rosette (flowerlike arrangement) made of 3 nanometer fibers D. a fully condensed metaphase chromosome 4) What is the key property of DNase that makes it useful for assessing whether chromatin is in a closed (tightly condensed) or open (loosely packed) configuration? A. DNase is a protein. B. DNase will digest DNAs from all species equally effectively. C. DNase preferentially digests DNA not associated with protein. D. DNase cuts at specific DNA sequences. 5) Ovalbumin, the major protein of egg white, is secreted by cells that line the oviduct as the immense egg or zygote (the unfertilized or fertilized egg yolk, respectively) moves down the oviduct. Imagine youre repeating the classic Weintraub-Groudine experiment, but with a twist: Youre assaying the DNase sensitivity of the promoter regions of the globin and ovalbumin genes in oviduct cells of laying hens. In this case you expect to find that: A. The globin and ovalbumin promoters are equally sensitive to DNase treatment. B. The globin and ovalbumin promoters are equally resistant to DNase treatment. C. The globin promoter is much more sensitive to DNase treatment. D. The ovalbumin promoter is much more sensitive to DNase treatment. 6) Imagine youve isolated a yeast mutant that contains histones resistant to acetylation. What phenotype do you predict for this mutant? A. The mutant will grow rapidly. B. The mutant will require galactose for growth. C. The mutant will show generally low levels of gene expression. D. The mutant will show generally high levels of gene expression. E. The mutant will show high levels of gene expression for only a few select genes. 7) Histone acetyl transferases exert their effect on gene activity by: A. neutralizing positive charges on lysines of histones B. increasing the negative charge on glutamic acids of histones C. influencing the sequence of the promoter D. increasing the affinity of transcriptional activators for DNA E. increasing the affinity of transcriptional inhibitors for DNA...
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This note was uploaded on 04/08/2008 for the course BIOL 203 taught by Professor Saha during the Spring '08 term at William & Mary.

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Chapter 18 Study Questions--answers at end - CHAPTER 18...

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