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Chapter 19 Study Questions--answers at end

Chapter 19 Study Questions--answers at end - CHAPTER 19...

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CHAPTER 19 STUDY QUESTIONS 1) Which of the following is critical to production of human growth hormone in E. coli ? A. The genetic code is universal. B. E. coli has a rapid growth rate. C. E. coli is a prokaryote rather than a eukaryote. D. Human growth hormone is a small protein. E. Human growth hormone purified from donated pituitaries sometimes is tainted with prions. 2) Why is E. coli so widely used as an organism in which to clone genes? A. E. coli has a rapid growth rate. B. Growing cultures of E. coli cells is easy. C. Researchers have extensive knowledge of E. coli genetics, biochemistry, and physiology. D. Many different E. coli strains with distinct and useful properties are available. E. All of the above answers apply. 3) Restriction enzymes are to bacteria what _________ is(are) to mammals. 4) Imagine you’ve isolated, directly from the human genome, the complete gene that encodes human growth hormone. After running through all the steps for cloning and expression described in Section 19.1 of this chapter, you find no human growth hormone is expressed. Why do you think this procedure failed? A. Human DNA cannot be cloned in a bacterium. B. Human DNA can be maintained in cloned form only for brief periods in bacteria. C. Bacteria cannot translate human mRNA. D. Bacteria cannot carry out splicing. E. Bacteria lack a nucleus for proper transcription of eukaryotic genes. 5) Genetic engineering techniques have developed by borrowing knowledge from unexpected sources. One such source was studies of retrovirus replication. What aspect of a common cloning procedure makes direct use of this knowledge? A. using restriction enzymes to cleave DNA B. synthesizing cDNAs C. introducing DNA into bacterial cells by transformation D. using retroviruses as vectors to carry genes into bacterial cells E. using plasmids to replicate many copies of a given gene within one cell 6) How does a gene library differ from a gene clone?
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