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BCHE 395-6 2013 Protein Motifs and Sequencing

Pep1de fragmenta1on cleave protein into smaller

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Unformatted text preview: monium Thioglycolate = Ammonium Thyglocolate Disulfide Bonds and Permanent Waves hKp://www.youtube.com/watch?v=dLdKU4JCYqg hKp://www.youtube.com/watch?v=_I_GYgGjxFM www.elmhurst.edu/~chm/vchembook/568hairwave.html 25 3. N- terminal sequencing: Edman Degrada1on •  Pehr Edman: two- step CHEMICAL process •  Labels and removes ONLY the amino- terminal residue from a [email protected] •  (1) Basic step: Phenylisothiocyanate (PCT) added under mildly alkaline condi1ons •  Reacts with amino- terminal aa to form a PCT adduct •  (2) Acidic step: Anhydrous trifl[email protected] acid cleaves pep1de bond next to PCT adduct •  Isolate and iden1fy the PCT adduct amino acid away from the shortened pep1de •  Repeat 26 You need to know the steps here, but not the mechanism (ie flow of electrons) Edman Degrada1on: N- terminal sequencing The am...
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