BCHE 395-9 2013 Ligand Binding and Hemoglobin

The conformanon change in the r state closes up the

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Unformatted text preview: hr Effect •  The carbamate forms addi<onal salt bridges stabilizing the T state à༎ promotes transi<on to T- State; ê༎O2 affinity CO2 binds to hemoglobin at amino- terminal residues LU NG [O2]é༎: [CO2]ê༎: pHé༎ OXYGENATED BLOOD (Hb) DEOXYGENATED BLOOD (Hb) CO2, produced as “waste” during respiraNon [O2]ê༎: [CO2]é༎: pHê༎ Tiss ues 20 pH Effect on O2 Binding to Hemoglobin •  Ac<vely metabolizing <ssues generate H+, lowering the pH of the blood near the Nssues relaNve to the lungs •  Hb Affinity for oxygen depends on the pH –  H+ binds to Hb and stabilizes the T state •  Protonates His146 (HC3!) which then forms a salt bridge with Asp94 •  Leads to the release of O2 (in the Nssues) •  The pH difference between lungs and metabolic Nssues increases efficiency of the O2 transport •  This is known as the Bohr effect Recall Thos...
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