BCHE 395-14 2013 Chymotrypsin Mechanism

Pg 216 lots of very good general informa6on you have

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Unformatted text preview: tly altered in the closely packed environment of a protein ?? Arg and His residues Mechanism •  (1) Sequence of steps (tracing the forma6on and breakage of intermediate interac6ons and bonds) •  (2) Structure of each intermediate and transi6on state •  (3) Rates of intermediate interconversion •  (4) Interac6on between enzyme and intermediates and transi6on states •  (5) Energe6c contribu6ons of all groups We know ALL SOME of this about very few enzymes, but the well- studied enzyme Chymotrypsin is best understood… 6 Learn to Read Reac6on Mechanisms! •  pg 216 •  Lots of very good general informa6on you HAVE TO KNOW •  HW #5!! 7 Catalyst’s Role Or roll… •  Major Mechanisms: •  (1) General acid/base catalysis –  Catalysis by proton transfer to “other” classes of molecules (most common rxn in biochemistry) –  Specific: acid- base catalysis using nonenzyma6c models à༎ H2O (H+/H3O+ and OH- ) –  By “other”, think amino acid side- groups in ac6ve sites •  (2) Covalent or Nucleophilic catalysis •  (3) Metal ion catalysis 8 General Acid/Base Catalysis Specific Acid/Base Catalysis Specific à༎ H2O as proton “donor” and “acceptor” More stable intermediateà༎ beler probability to go to product (rate of reac3on increases) (rate of reac3on then slowed) ß།General Acids (HA) and bases (B) as proton “donors” and “acceptors” Nonenzyma6c Condi6ons/ Reac6ons Using water or weak acids/weak bases as models 9 Amino Acids in General Acid- Base Catalysis 10 Nucleophilic or...
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