Lecture notes 2

8 kcalmol 5 succinyl coa synthetase 1 kcalmole

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Unformatted text preview: of E2 [(E1)2]12 *E3’s assemble in dimeric way on each of the six faces of E2 each:[(E3)2]6 • The 24 dehdrogenase proteins (E1) on the outside of the complex bind pyruvate and release CO2 • The 24 transacetylases (E2) proteins shujle remaining atoms to CoA and the electrons to E3, • The 12 dehydrogenases E3 takes electrons from E2 through the FAD and ulFmately to NAD+ Blow up of one E2 To what end? 1.  2.  3.  4.  AcFve aldehyde Aceyllipoamide Reduced/oxidized lipoamide Reduced/oxidized FAD Rxns TCA cycle •  1. Citrate synthase: –   ­ 8 kcal/mol •  2. Aconitase: –  + 3 kcal/mol •  3. Isocitrate dehyd.: –   ­ 5 kcal/mole •  4. α ­ketoglutarate dehyd.: –   ­8 kcal/mol •  5. Succinyl CoA synthetase: –   ­1 kcal/mole •  6. Succinate dehyd: –  0 kcal/mol •  7. Fumarase: –   ­1 kcal/mol •  8. Malate dehydro.: –  +7.5 kcal/mol Some bookkeeping... •  The overall reaction - The net reaction of the cycle is Acetyl CoA + 3NAD+ + FAD + GDP + Pi + 2H20→ 2CO2 + 3 NADH + FADH2 + GTP + 2H+ + CoA 2 carbons in Oxidn state -CH3 - 3 -COS +3 --------------0 Overall: 2 carbons out Oxidn state CO2 2 x (+4) -----------+8 8 e- oxidation All e- leave in hydrogenations: FAD → FADH2 (2 e-) 3 NAD+ → 3NADH (3 x 2 e-) What is the significance of the “cycle”? •  All intermediates are “cofactors” in a broad sense –  That fact was how the cycle was discovered...
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