Bioinformatics - Bioinformatics November 7, 2007 Monday...

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Bioinformatics November 7, 2007 Monday Night Purpose: The purposes of this lab were to make a gel, load DNA onto the gel, and see the DNA fragments using electrophoresis, and to learn about Bioinformatics using the NCBI web site. Hypothesis: Restriction enzymes can cut DNA into fragments. Methods: 1. Tape the sides of a gel tray, place the comb in the slits atop the tray, and place the prepared tray into your green tray. 2. Use the provided flask to make an agarose solution. Mix .25g of agarose with 25ml of 1X electrophoresis buffer; allow to hydrate for one minute, then swirl gently to form a suspension 3. Microwave for about 1 minute. Allow the flask to cool, then pour the melted agarose into the casting tray. Allow to set for 15 minutes 4. Remove the comb from the chamber, remove the tape from the gel tray, and transfer the tray to an electrophoresis chamber so that the sample wells are nearest the negative electrode. 5.
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This note was uploaded on 04/08/2008 for the course BIO 101L taught by Professor Culp during the Fall '07 term at Canisius College.

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Bioinformatics - Bioinformatics November 7, 2007 Monday...

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