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Unformatted text preview: Fermentation Monday Night Pu rpose: The purpose of this experiment was to observe the effects of different carbohydrates on the fermentation of yeast. Hypotheses: 1. Starch is a substrate for yeast fermentation. 2. Lactose +Lactase is a better substrate for yeast fermentation than lactose alone. Methods: 1. Tape label each beaker to be used in the experiment, and measure 35 ml of the sugar solutions and/ or carbohydrate and enzyme combinations and pour them into the beakers 2. Add 0.5 g yeast to each beaker, stir, and wait 5 minutes. 3. Pour the contents into the corresponding numbered fermentation tubes. Carefully invert each tube so the “tall” portion is filled with solution. 4. Place the tubes in a 37 C incubator. At intervals of 20, 40, and 60 minutes ⁰ from the start of the experiment measure the distance from tube tip to fluid level and record results in table 1 . 5. Calculate the volume of gas evolved using the formula: V= πr2h . Then convert volume from mm 3 to cm 3 and graph (you will now refer to the volume of gas generated as the volume of the specific type of gas produced ....
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This note was uploaded on 04/08/2008 for the course BIO 101L taught by Professor Culp during the Fall '07 term at Canisius College.
- Fall '07