biomaterials hw 3 complete

We can find the same information for the bsa protein

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Unformatted text preview: egression. 1/Γ=[1/(Γ!"# *!! )](1/[P])+1/Γ!"# . This shows us that for Cyt ­c 1/Γ!"# =0.010 and 1/(Γ!"# *!! )=6.419. We then find that Γ!"# =100 and !! =0.001558 for Cyt ­c according to the Lineweaver ­Burk model. We can find the same information for the BSA protein. We see that 1/Γ!"# =0.004 and 1/(Γ!"# *!! ) =15.58. The Lineweaver ­Burk regression concludes that BSA has a Γ!"# =250 and !! =0.000257. BSA Lineweaver ­Burk 1/[Γ] (mg MS/μg protein) 0.18 0.16 y = 15.549x  ­ 0.0039 0.14 0.12 0.1 0.08 Series1 0.06 Linear (Series1) 0.04 0.02 0 0 0.002 0.004 0.006 0.008 0.01 0.012 1/[P] (ml/μg) b.)The Lineweaver ­Burk model is very sensitive to error while the Langmuir model has little sensitivity. The Langmuir regression is biased toward middle and higher concentrations hilte the Lineweaver ­Burk model is more biased toward lower concentrations. We see that the !! values are a whole order of magnitude smaller when found using the Lineweaver ­Burk model. Since the concentrations we have are not that high, the Lineweaver –Burk model is probably better. c.) Hydrodynamic radius of Cyt ­c: !! =0.18 nm 1 KDa= 1.66×10!!"...
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This document was uploaded on 03/05/2014 for the course BMEN 3400 at Tulane.

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