Name: Gabriela Gaytan Section: 111-522 1 Heredity I Lab Objective1.Describe the inheritance pattern of monohybrid and dihybrid crosses in Arabidopsis thaliana. Safety 1.Closed-toe shoes must be worn. 2.Long hair tied back. 3.Goggles must be worn at all times while experiments are being conducted in the classroom. Even if you’re done. 4.Gloves should be worn while performing the experiments. 5.All pipet tips should be ejected into the tip waste bin on your bench. 6.Green filter goggles must be worn when examining plants under royal blue light. Lab Activity 3: Heredity in a Dihybrid Cross Prep Staff has grown flats of both parental types for you: There is a photo of older plants in the pre-lab online.
Name: Gabriela Gaytan Section: 111-522 2 1.Observe these close-up images of the plants of each of the homozygous parental lines gfp1 and gl1 and noteany differences you see below. Visible differences between gfp1and gl1 plants: The gfp1plant is hairy (has trichomes) while the gl1plant is glabrous. 2.Study the images of the seedlings of each of the true-breeding parental lines, gl1 and gfp1, taken under the dissecting microscope. Pay close attention to the first true leaves. Note your observations below. Homozygous for gl1Homozygous for gfp1Observations: Homozygous for gfp1has trichomes while homozygous for gl1is smooth.
Name: Gabriela Gaytan Section: 111-522 3 3.Observe photographs of plates of seedlings taken under royal blue light illumination a darkened room. These images were taken through green filter goggles using only the blue light for illumination. Under this light, plants expressing gfp are visible in good detail and appear green. Plants not expressing gfp appear only as indistinct dark shapes. (You may see some backscatter from the black surface under the plants.) Thus, if you can see it clearly, a seedling is glowing.