Final Review P1

Your name q1b 8 points consider

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Unformatted text preview: arallel coiled coil with Helix B. 2. Neither helix forms homodimeric coiled coils (i.e., A-A and B-B coiled coils do not form). 3. Each helix contains two each of E and K. You can ignore the possibility of forming antiparallel coiled coils. (i) Write your designed sequences, using single letter codes, in the boxes below. (4 points) a b c d e f g a b c d e Sequence A: Sequence B: (ii) (2 points) Explain briefly why your sequence ensures heterodimerization (A-B) without homodimerization. (2 points) f g John Kuriyan UC Berkeley, Chem C130/MCB 100A, Fall 2010, Mid-term Exam 1. Your Name___________________________ Q4. (20 points) Q4A. (10 points) Shown below is a schematic diagram of two helices forming a parallel coiled coil. Consider two small proteins, A and B, of 14 residues each that are made up of only the following four amino acids: L, Q, E, K. Design sequences for A and B so that the helices obey the following rules: 1. Helix A forms a parallel coiled coil with Helix B. 2. Neither helix forms homodimeric coiled coils (i.e., A-A and B-B coiled coils do not form). 3. Each helix contains two each of E and K. You can ignore the possibility of forming antiparallel coiled coils. (i) Write your designed sequences, using single letter codes, in the boxes below. (4 points) a b c d e f g a b c d e f g Sequence A: Sequence B: (ii) (2 points) Explain briefly why your sequence ensures heterodimerization (A-B) without homodimerization. (2 points) (iii) (4 points) The pKA values of the histidine residues in mixtures of these proteins is measured, and is found to be altered with respect to the normal pKA of histidine. Circle the most likely value of the pKA. Explain your choice. A. pKA=5.0 B. pKA=6.5 C. pKA=8.0...
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This note was uploaded on 03/05/2014 for the course MCB 100A taught by Professor Kuryian during the Fall '09 term at Berkeley.

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