New Perspectives on Melanoma_ The Role of PAX3

81 rnaextractionandrtqpcr

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Unformatted text preview: .......................................................................................................... 78 Introduction ......................................................................................................................... 79 Materials and Methods .................................................................................................... 80 Cell culture ................................................................................................................ 80 . Immunocytochemistry ......................................................................................... 80 Western blotting ..................................................................................................... 81 ChIP ............................................................................................................................. 81 qPCR analysis of the gene promoter .............................................................. 81 RNA extraction and RT‐qPCR ............................................................................ 82 Statistical analyses ................................................................................................. 82 Results .............................................................................................................................. 83 PAX3 expression in HEM1455 primary melanocytes and A2058 melanoma cells ............................................................................................ 83 . PAX3 target genes in HEM1455 and A2058 cell lines ............................ 84 Expression of PAX3 targets in HEM1455 and A2058 ............................. 86 Discussion ............................................................................................................................. 87 Acknowledgments ............................................................................................................. 91 Supplementary data ......................................
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