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Unformatted text preview: er. photons-retinal experiment
inject LCMV in the head of the mouse's brain. once it recovers from this invasion, it is sacrificed and then spleen cells are taken out mostly
lymphocytes. purify it as a flow
cytometry using cd8 purifier
the cells must be infected by the virus.
then present the viral proteins
the superscript k is haplocyte
target cells can be be bought
the spleen cells are not sensitized and thus the
haplotype is responsible.
Kimball _3rd edition Intro to Immunology
C3H.OH shows that cytotoxic and the mhc to be
the same for it to match. if the class 2 matches up then it will get a DTH response but if it does not match up then it doesnt cause a response. allogeneic(different in
everyplace, different alleles) the DTH response is a mhc class 2 restricted phenomenon. one of the the IE matches but the other doesnt even though
it created a response because it only needs one heterodimer to match up and the IA is similar. the beta but not the alpha are similar but not enough Important Kimball _3rd edition Intro to Immunology Use Fowl Gamma Globulin to set up DTH assay (lymphocytes adoptively
transferred from FGG exposed donors into naïve recipi...
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- Fall '12