Areas of elongation are apparent after ber has

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Unformatted text preview: and in water. The fiber was observed under rizing light with a 530-nm first-order red . Areas of elongation are apparent after fiber has undergone mechanical testing. 3 4 x 1 2 3 3 were nsignalcsequencehe break point (Fig. ot asso iated with t in microsurgery or in uses requiring a high directs protein into 6E). This phenomenon resembles the deformalevel of energy absorption and elongation tion besavior of polyethylenes in which chains h ecretory pathway similar to that provided by Nylon (37, 40).pA addition site from In are pulled into the “elongated” area and a contrast to the manufacture of aramid fibers, growth hormone cow “necking” is propagated along the fiber (38). requires dragline silk protein a process that production of rc– spider silk coding sequence highly concentrated (BGH) gene Scanning electron microscopy (SEM) verisulfuric acid, fied the ability to spin solid core fibers using using water as the solvent offers obvious Polarizing light our aque enhancer and promoterous process. A scanning electron mi- advantages (40). An additional benefit is that crograph of an as-spun ADF-3 fiber stored at silk will ultimately degrade in aqueous envifrom cytomegalovirusmidity is shown in Fig. 7. The figure ronments, providing a “green” alternative to 50% hu shows the cylindrical, smooth surface of the traditional synthetic fibers (40). 6 3 Downloaded from on March 2, 2008 3 Downloaded from on March 2, R1 IRMOF 1 I OF-3 u d t a 21.490(1) Å, V 9925(1) free R -BDC acid. ing a pattern of alternating Ala-rich, crystalwe generated two series of constructs for icngtionalw-heraendheRMptake wns efrouhe tso msilk synthesis, the Å , Z 1, R1 0.1845. During be [See (23) for details.] onditions machinery. a t ir u nd e 11 October 2001; accepted 6 December 2001 forming blocks (ASAAAAAA blocks) that expression of rc– spider silk proteins in mam- lower [135 and 120 cm (STP)/cmgland-specific O’Keeffe, B. G. Hyde, Crystal Structures I: Patterns spider produces ] than that of 13. M. pools of IRMOF-6 —a significant difference that is atimpart the silk’s mechanical properties (14– malian cells using spider dragline silk gene tribtRNAsthfor drglycine tuand C H utable to e hy ophobic na re of alanine to meet the ts in IRMOF Thus, fun for limiting amino acids 16 ) and Gly-rich amorphous blocks cDNAs: the MaSpI or MaSpII cDNAs (12) uniincreased-6.demandctionalizing the Spider Silk Fibers Spun from a carb ted in Example: silks are implicated in pro- and the ADF-3 cDNA super-tough In por(esOwit4h l5r,gaenrdh-y7d, rotheons as illustraamino [GGYGPG, (GPGQQ)n],secreted proteins that assemble into (11, 32) (Fig. 1). bersRM33-)., -Given may indeed result in even acid composiunique I F her c aci s. T mos open memb (e.g., viding elasticity in the silk filament (17 ). The addition, expression vectors were generated higtionapoftiethehesilk t proteinsers of MaSpII: Soluble Recombinant Silk 32% this series (IRMOF-12 and -14) are also porous, process by which spiders spin soluble silk containing multimers of the dragline cDNAs in glycine,xhibit behal...
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This document was uploaded on 03/08/2014 for the course BIOC 406 at University of Washington.

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