Al tra sform ion n a 24 possible to generate with

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: y p strct ud 38). I s o 110 a rs p 140 s raw on diameter and tensile strength sug- has been reported positively correlated rediuctere (sizen c( ntrast, spidend roduce silkkD) (Fig. 2A, s that it should be). Several groups have attempted to spin in an as r ult o ap Fig. al tra sform ion n a (24 possible to generate with molecular orientation, resulting lanesa3esandf r4;id physic2B, nlaneat2) iproduced from 472 precipitated similarly couldJANUARY e redissolved SCIENCE 18 only b 2002 VOL 295 s similar to native dragline silk with increase in the proportion of the -sheet cryssingle processing step in parallel with drawing in strong denaturing solvents such as hexaflusilk protein fibers artificially. Most efforts (36concatemers iofintheaw is precludgene 7(ADF-33 and talline areas and amorphous domains , because postsp nn g dr ADF-3 ed (7, 3 , 37). A transition of oroisopropanol or guanidine hydrochloride (23). have involved wet spinning ADF-3birefringence was shown 39a)r. prhecessindneisig npaeamlteitngsinllfof rfMaSpI, respectiveof reconstituted fibers ul T o sp and ar su er a obe oora mtoon ADF-333) n g r dimer i w f r ma i dB between the His and ADF-3 This differencTheolubility may be attributed to e in s secretory pathway Bombyx mori (25–27 ) orBnative ADF-3 Hissilk were diversall stinstances,ommon different expression spider fibers producedtiinwcultured acells Bioengineering:6,synthetic silks ly. In ity ar ng ith a c the prim ry silk (Fig. and D). brittle, fibers demonstrated protei sequen . I i at a by va ythe presence of the COOH-terminus in ADF-3 (28, 29). Recombinant whereas proteins have silk ADF-3 properties. vectorsneusedciematrys saenqtiuceinceesd atnsecretion of soluble enabledpthe hdt spinnirng appreciable mechanical ing th silk pr and MaSpII rc– spider silk proteins produced in been spun with harshers solventsicrosuchallyaster mesilkparameters, fibers the a range of Distinct spider silk proteins in with media. specificaFib observed m scopic af chanical testing were generally uniform with tions can be 37). Recombinant DNA (30) as diluents or dimammalian cells, suggesting that the more hyhexafluoroisopropanoltinconstruct: ngation. These elongateproteins withproduced (of applications: for 120, 150, molecularThese fibers sizes of dis ct areas of elo d could be used in a variety cytoplasm a as concentrated ter decre d example, and fine kD have sutures drophilic COOH-terminal 100 amino acids [ablute D protein solutionsrein showed a 12% diameformicase an190, 250, as very750 monofilament been found in the 26 protein folding endoplasmic reticulum (ER) glycosylation formation of disul de (—S-S—) bonds between pairs of cysteine residues quality control (veri cation of folding) packaging into vesicles for transport to Golgi cytoplasm Fig. 7. Scanning electron micrographs of an ADF-3 as-spun fiber. (A) Analysis of fiber surface (magnification, 500); (B) analysis at a break point to examine the fiber interior core ( 2000). SCIENCE VOL 295 18 JA...
View Full Document

{[ snackBarMessage ]}

Ask a homework question - tutors are online