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Unformatted text preview: high protein conpro i
u J. Solid State ht 48 , 1 (2000).
inability to domesticate spiders have driven
MaSpI, andsilk secretions, onstructs by Western blot 2. erJ.M.molecD.., Ward, Sci. eig267., 152(1992).r silk protetienns(.ADF-3e60 kilodaltons) under modest shear and coagulation conditions. The
Yaghi et al lar w Chem spide
centration, and low spinning speeds (7 ). It
spun ﬁbers were water insoluble with a ﬁne diameter (10 to 40 micrometers) and
M. Eddaoudi al.,
o t276,Res. o319 (2001).
numerous attempts to artificially manufacture
(32) revealed pthoughtnentthemmunoreactive bands 4. apA. earaet ceAcc.fChem. s 57534, tein “ladder” whisitpd oobahness and modulus values comparable to those of native dragline
was previously romi that i silk protein
5. V. pRussell n al., Science hi pr(1997).
exab e r t ug 6. Y. H. Kiang et al., J. Am. Chem. Soc. 121, 8204 (1999).
the primary factor
s ln s t w h o
b.lyHdsune, Rt.oobtsenr, minahteim. nc.e1rrors2 of proteiiks buyntithel-wer tenacity. Dope solutions with rc– silk protein concentrations
spider dragline silks for industrial and mediof sequence would bebreak (J/kg) deter2) the expected molecular size (Fig. 2A, lanes 1,
7. B F. o ki s
R o J. Am. C o So
mining fiber properties. We spun fibers from
20% and postspinning draw were necessary to achieve improved mechanical
n t et of t
cal applications. Recombinant (rc)– spider
2, and(ADF-3) iof .the B, proteins 1). To explore the 8. s(F.is8.9).TranFerey,tJ. onalChem. u,sing, resultingprioperhies eroh-e spun ﬁbers. Fiber properties correlated with ﬁner ﬁber diameter
one 5; F g 2two lane present in
Serpaggi, G. sla i Mater. pa 8 2749 (1998).
Dragline silk in bacteria 9 reladraglinebetw1ex 105dermass k 60rkDein size and 9. geLi et ouNaturer402e276 (1999). ression, has beend rncpeased direfringence.
1 x 10
silk with a molecular
an i e r orte b
ne s adjective, deﬁned in
silk proteins have been produced
e n spi
sil p ot
10. “Reticular” is anp ot in exp Random House
(1) and mechanical properties approaching
iWebster netlike.” ipes (16,as22as “having the . morevolutionary survival of spiders has been glands, each producing silk with different
n net; Unabridged Dictionary “having the form The i (34).
and yeast systems with limited success4(x 109 mechanof al p3 x ertdraglinefsilk. un fibers, we first
those ic nativerop 10es o sp This respider i4
of a N. clavIsoreticular is deﬁned ) and B
tightly linked d c ld tio 1– ). Orb-web production, and Dragline silk, used as and functions (4– ).
22). The highly repetitive structure and xthe 6 evaluasecondthe questiony4of the mammalian epithe- 11. sameADF-3 formulatedeas ovO(Link) (DEF) m conofisilksn(eto3theudiversity,spinning spiders mechanical properties the safety line and6as
ted the 8bi10 the contributionthe
a x lit of or MaSpI, to of
All IRMOFs were was r c Zn ered fro .
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