Erjmmolecd ward sci eig267 1521992r silk

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: high protein conpro i u J. Solid State ht 48 , 1 (2000). Th; inability to domesticate spiders have driven MaSpI, andsilk secretions, onstructs by Western blot 2. erJ.M.molecD.., Ward, Sci. eig267., 152(1992).r silk protetienns(.ADF-3e60 kilodaltons) under modest shear and coagulation conditions. The 3. O. Yaghi et al lar w Chem spide centration, and low spinning speeds (7 ). It spun fibers were water insoluble with a fine diameter (10 to 40 micrometers) and M. Eddaoudi al., o t276,Res. o319 (2001). numerous attempts to artificially manufacture (32) revealed pthoughtnentthemmunoreactive bands 4. apA. earaet ceAcc.fChem. s 57534, tein “ladder” whisitpd oobahness and modulus values comparable to those of native dragline was previously romi that i silk protein 5. V. pRussell n al., Science hi pr(1997). et exab e r t ug 6. Y. H. Kiang et al., J. Am. Chem. Soc. 121, 8204 (1999). the primary factor s ln s t w h o b.lyHdsune, Rt.oobtsenr, minahteim. nc.e1rrors2 of proteiiks buyntithel-wer tenacity. Dope solutions with rc– silk protein concentrations spider dragline silks for industrial and mediof sequence would bebreak (J/kg) deter2) the expected molecular size (Fig. 2A, lanes 1, 7. B F. o ki s R o J. Am. C o So 11, 596 Material Strength (N/m Energy to mining fiber properties. We spun fibers from 20% and postspinning draw were necessary to achieve improved mechanical 19 n t et of t cal applications. Recombinant (rc)– spider 2, and(ADF-3) iof .the B, proteins 1). To explore the 8. s(F.is8.9).TranFerey,tJ. onalChem. u,sing, resultingprioperhies eroh-e spun fibers. Fiber properties correlated with finer fiber diameter one 5; F g 2two lane present in Serpaggi, G. sla i Mater. pa 8 2749 (1998). ´ H. al., , Dragline silk in bacteria 9 reladraglinebetw1ex 105dermass k 60rkDein size and 9. geLi et ouNaturer402e276 (1999). ression, has beend rncpeased direfringence. 1 x 10 silk with a molecular of an i e r orte b ne s adjective, defined in silk proteins have been produced tion e n spi sil p ot 10. “Reticular” is anp ot in exp Random House (1) and mechanical properties approaching iWebster netlike.” ipes (16,as22as “having the . morevolutionary survival of spiders has been glands, each producing silk with different n net; Unabridged Dictionary “having the form The i (34). and yeast systems with limited success4(x 109 mechanof al p3 x ertdraglinefsilk. un fibers, we first 18– those ic nativerop 10es o sp This respider i4 Kevlar of a N. clavIsoreticular is defined ) and B network topology.” sult raises tightly linked d c ld tio 1– ). Orb-web production, and Dragline silk, used as and functions (4– ). 22). The highly repetitive structure and xthe 6 evaluasecondthe questiony4of the mammalian epithe- 11. sameADF-3 formulatedeas ovO(Link) (DEF) m conofisilksn(eto3theudiversity,spinning spiders mechanical properties the safety line and6as ted the 8bi10 the contributionthe a x lit of or MaSpI, to of All IRMOFs were was r c Zn ered fro . the protein, A...
View Full Document

Ask a homework question - tutors are online