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[See (23) for details of elemental analyses.]
u the intensity b were collected a on have as to as %
unusual mRNA secondary structure result in
lial silk’sls toproperties.ce rc– spider silk proteins of 12.tAllre mediadata y precipitontiBruker with 15many 20seven highly specialized the frame thread of the spider’s web, is one of
cel fiber produ
5. Stress-strain curves from testing of ADF-3– based ﬁbers (sample 1, Table 1). 109
1 x The curves igh modieicnslunrxrweichhhe simiarerspto that of silk
the strongest silks studied, being three times
ammonidiffractometerawithÅ) or an mono-ichment of at least
u ( 0.71073 a graphite Struch andCprn cesoed afore ewahmglet tbyfibeaswlng) aurn
sent analysis inefficient translation fragments, each 1/2 inchsize of
performed on seven different ﬁber that limits the in length.
chromated MoKm sulf te f radiation. enr
ol s u (
dr i a
as tough as aramid fibers and five times
tures were solved by direct methods, and successive
cr t c l o d se mi ed in n c
r id r s.
50% in a ssyntheses step (32). The pJ7V cipBiotechnologies, -Vaudreuil-Dorion,U.S. Army stronger by weight than steel (7–10).
re 8P5, itated Materials Science Team, Quebec
the silks produced (23). The feasibility of
proteiiinas in beterrvning mechtahieal sppopeetire’s silk glands
difference Fourier ingle with SHELXTL software
s into sheetsD. Tirrell, tenacity values
(29). The 1996. Sciencefurther optimization of the spinning process.
Wet spinning of rc-silk as described here de271:39
package. Final R1 values were calculated with I
t2 i(nsCrystalodataaare infollows: DF-3, cubic,ereSoldieraUSA.y redis- Command, Natick, MA secretedprotein core of dragline silk fibers is
e I). , c nt inas g A IRMOF-2, w 01760, Biological Chemical
( pe Aon a chem o t c forma iin and s eral
– spider silk fibers was lower than that of rc– spider silks (up to 100 spun23). nds nalysisical fransondittoonedevmedia revealed
A decrease in the diameter of the
as a mixture of two soluble proteins
space group Fm-3m, a 25.772(1) Å, V 17117(1)
h p://www.sciencenews.org/pages/sn_edpik/ps_5.htm the ppsrtesnnncedrow stcps sfpr dehieviinlg fiperoteins of the
e dragline silk; however, from this and
fiber with higher draw correlatedin
with ino spi e ing a f r e – oi ac r s k b r
sÅo,lZve8, R1n 0.0976;sIRMOF-3,ecubic, Fm-3mead sal*Toewhom correspondence should be addressed. E- from specialized columnar epithelial cells of
d i pho phat -buffer ,
in . In contrast,
kD) by using synthetic genes (MaSpI)
r studies (29), h p://www.naturalhistorymag.com/1006/1006_biomechanics.html esumably due to aligned microcomparison of the effects
creased birefringence (Fig. 6). Upon drawing,
mail: [email protected]
25.747(1) Å, V 17067(2) Å , Z 8, R1 0.1160;
the major ampullate gland of orb-weaver
rc– spider silks produced in E. coli or yeast and
transgenic plants birefringence in silks is recentl...
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This document was uploaded on 03/08/2014 for the course BIOC 406 at University of Washington.
- Winter '14