When it nds the complementary strand it ac4vates

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Unformatted text preview: ific RNA ­binding proteins and transla4on control Sequence ­specific RNA ­binding proteins and transla4on control RNA interference (RNAi) • RNA interference (RNAi) is a mechanism that inhibits gene expression by RNA molecules - post-transcriptional level - transcriptional level RNAi machinery •  Dicer: an endoribonuclease in the RNase III family •  Argonaute •  RNA ­dependent RNA polymerase (RdRp) RISC complex (RNA ­induced silencing complex) • It is a mul4 ­protein complex which cleaves (incoming viral) dsRNA and binds short an4sense RNA strands which are then able to bind complementary strands. • When it finds the complementary strand, it ac4vates RNAse ac4vity and cleaves the RNA. • Known components are: Argonaute proteins, and various RNA binding proteins. • The Argonaute proteins, AGO1 and AGO2 contain a PAZ domain (a conserved domain present in AGO proteins that binds to small RNA helices) as well a...
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