Post_transcriptionalRegulation

When it nds the complementary strand it ac4vates

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: ific RNA ­binding proteins and transla4on control Sequence ­specific RNA ­binding proteins and transla4on control RNA interference (RNAi) • RNA interference (RNAi) is a mechanism that inhibits gene expression by RNA molecules - post-transcriptional level - transcriptional level RNAi machinery •  Dicer: an endoribonuclease in the RNase III family •  Argonaute •  RNA ­dependent RNA polymerase (RdRp) RISC complex (RNA ­induced silencing complex) • It is a mul4 ­protein complex which cleaves (incoming viral) dsRNA and binds short an4sense RNA strands which are then able to bind complementary strands. • When it finds the complementary strand, it ac4vates RNAse ac4vity and cleaves the RNA. • Known components are: Argonaute proteins, and various RNA binding proteins. • The Argonaute proteins, AGO1 and AGO2 contain a PAZ domain (a conserved domain present in AGO proteins that binds to small RNA helices) as well a...
View Full Document

Ask a homework question - tutors are online