BiocoreIISignaling2014preclass

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Unformatted text preview: dize probe to @ssue Each cell that contains mRNA of interest will stain blue 38 Intriguing Expression Pa;ern Unpaired in Drosophila plays the same role as Prolac@n in vertebrates What is the possible role of Upd? How would you test it? Hub cell Tulina and Matunis (2001) 39 GFP markers of cell iden@ty Gene x in situ hybridiza@on Regulatory region of gene x GFP DNA organism (transgenics) Why might it not match the in- situ hybridiza@on? Upd func@on WT Upd mutant Germ line stem cell marker Hub cell marker spermatogonia marker Does unpaired work cell autonomously or non- cell autonomously? 41 Evidence of Func@on •  Necessary – the gene is required for a par@cular func@on Loss of func@on •  Sufficient – the gene alone is sufficient to impart a par@cular func@on Gain of func@on 42 What happens with too much Upd signaling? morphological markers Upd ectopic overexpression WT A DNA stain marks stem cells and spermat- ogonia “ectopic” - - in a loca@on where the en@ty is not normally found Spermat- ocytes are weakly staining Hub Branched fusome spermatogonia Spherical fusome – stem cell marker 43 Tulina and Matunis 2001 JAK- STAT pathway in Drosophila Testes Hub cell Upd Unpaired (Upd) Hopscotch (Hop) Stem cell Stat92E En@re stem cell transcrip@onal program 44 •  Juxtacrine signaling 45 Juxtacrine Signaling in Vulval Development in Caenorhabditis elegans 3º 2º 1º 2º 3º 46 Private Conversa@ons in Development I am primary fate. You are not! Okay, I hear you. Commonly called lateral inhibi@on 47 Delta- Notch – private line of communica@on P6.p P5.p 1.  Notch binds Delta on an adjacent cell 2.  ADAM10 cleaves N...
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