BiocoreIISignaling2014preclass

Cullin skp f box degradaon domains i ii iii relave

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Unformatted text preview: otch on the outside of cell 3.  The intracellular fragment of Notch is released to the cytoplasm 4.  Notch interacts with transcrip@on factors in the nucleus to mediate transcrip@on 48 What are some general proper@es you can extract from these different signaling pathways? •  •  •  •  Signal starts from outside the cell Signal ends in the nucleus Signal typically involves many steps Typically relies on repressing a repressor •  What could be the role of having many steps? 49 Brief break 50 51 •  Gravitropism – turning or movement in response to gravity 52 Major plant hormones Cytokinin – cell division, shoot meristem forma@on Gibberellic Acid –seed dormancy, flowering, growth Jasmonic Acid – pathogen defense responses and stamen development Ethylene – defense responses, fruit ripening Brassinosteroids – Light induced developmental changes Salicylic Acid – pathogen response Auxin – bending to light and gravity (tropisms) and many developmental responses 53 Auxin Signaling tryptophan auxin vascular development gravitropism cell fate specifica@on lateral root forma@on organogenesis cell elonga@on – in the root, it inhibits cell elonga@on •  stem cell specifica@on •  •  •  •  •  •  54 Big Ques@on: What Are the Mechanisms of Auxin Signal Transduc@on auxin ? Auxin Responses 55 • Auxin mediated phenomena such as bending toward gravity involve massive developmental changes that require the transcrip@on of suites of genes. What kinds of proteins do you think are involved? 56 Environmental or developmental signal Transcrip@onTransla@on Transcrip@onTransla@on Transcrip@on Factor 57 Auxin Percep@on & Response the last step Transcrip@on factor Auxin responses Auxin Response Factors (ARFs) There are 23 in Arabidopsis 58 Auxin Percep@on & Response •  ARFS (transcrip@on factors) bind to DNA to induce transcrip@on •  ARFs appear to be cons@tu@vely expressed in specific cells •  AUX/IAAs repress ARFs by binding directly to them AUX/IAA blocked X Auxin responses Auxin Response Factors (ARFs) Transcrip@on factor 59 Auxin Affects Aux/IAA Proteins Experiment:...
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This document was uploaded on 03/09/2014 for the course BIOLOGY biocore at NYU.

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