BiocoreIISignaling2014preclass

How would you test it hub cell tulina and matunis 2001

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Unformatted text preview: ct and uterus and the boundary 30 DES Model estrogen Endocrine signal Estrogen Receptor “agonist” disrup@on Estrogen Receptor Paracrine signal Agonist – fully or hyper excites receptor – It can s@mulate or block signaling Antagonist – blocks signaling 31 Hedgehog – relief from degrada@on II on state off state Patched 1. Hedgehog binds Smoothened 2. Patched is receptor, Patched inhibits Smoothened in off state 3. Hedgehog binding ac@vates Smoothened, which then represses PKA and Slimb 4. CI is no longer cleaved by PKA and Slimb and can ac@vate transcrip@on JAK- STAT – relay of dimers 1. Ligand is prolac@n or in Drosophila, Unpaired. 2. Prolac@n receptors form a homodimer 3. Receptors bind to Jak2 on the cytoplasmic side of cell and are phosphorylated 4. Jak2 phosphorylates Stat5 A very important signal in maintaining stem cells 5. Phosphorlyated Stat can homodimerize, traffic to nucleus, and mediate new transcrip@on 33 JAK- STAT signaling in stem cell maintenance in Drosophila 34 •  What is a stem cell? •  What is a stem cell niche? 35 How are adult stem cells maintained? stem cell niche niche – mul@cellular “environment” stem cells •  What kind of signaling is involved suppor@ve cell 36 Drosophila testes niche suppor@ve cell Cell division – “transit amplifica@on” A cell type proliferates itself 37 Technique: In Situ Hybridizaton 1. design probe of interest UACG…………………….. AUGC…………………….. AAAAA mRNA of interest 2. prepare @ssue of interest 3. hybri...
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This document was uploaded on 03/09/2014 for the course BIOLOGY biocore at NYU.

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