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In gas chromatography the retention factor is varied

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Unformatted text preview: raphy, the retention factor is varied by changing the column temperature during the run (temperature programming). In liquid chromatography, the retention factor is varied by changing the composition of the mobile phase during the run (gradient elution). 8. The number of plates in a column for a given analyte can be determined by measuring the retention time (tR) and the width of a peak at its base (W) or its half height (W½). The number of plates is then given by: 9. Decreasing the peak widths will increase resolution as will increasing the peak separation. Homework Solutions...
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