Unformatted text preview: raphy, the retention factor is varied by changing the column
temperature during the run (temperature programming). In liquid
chromatography, the retention factor is varied by changing the composition of the
mobile phase during the run (gradient elution). 8. The number of plates in a column for a given analyte can be determined by
measuring the retention time (tR) and the width of a peak at its base (W) or its
half height (W½). The number of plates is then given by: 9. Decreasing the peak widths will increase resolution as will increasing the peak
separation. Homework Solutions...
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