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Unformatted text preview: Problem Set 4 Name: Genetics 0350 Martens Due February 28, 2008 at 2:30 pm 15 points total 1) The GAL1 gene encodes an enzyme that is required for yeast to use galactose as an alternative carbon source when glucose is limiting. You have isolated a spontaneous mutant yeast strain that can no longer metabolize galactose. You then treat this mutant with proflavin, a mutagen that normally generates an insertion or deletion of a single nucleotide, and select an intragenic suppressor. You hypothesize that the suppressor strain contains a single base pair insertion and a single base pair deletion in the GAL1 gene that are compensating for each other. Subsequent analysis reveals that the suppressor strain has several amino acid changes in the Gal1 protein as compared to the original strain as shown below. a) 6 points. Is the genetic code consistent with your hypothesis of a single base pair insertion and deletion in this gene. Demonstrate this by indicating all the possible codons for each amino acid and deletion in this gene....
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- Spring '08
- DNA, Leu Thr Ala Thr Leu His Ala Ser Leu Ala, AUU AUC AUA, GUU GUC GUA, GUC GUA GUG