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Unformatted text preview: n, light, fluorescence) Separation (e.g., centrifugation, gel filtration, PAGE, Fluorescence activated cell sorting (FACS)) Immunological (e.g., Western Blotting, immunohistochemistry, immunofluorescence) B. Genetics and Molecular Biology (16-17%) 1. Biological compounds Macromolecular structure and bonding Abiotic origin of biological molecules 2. Enzyme activity, receptor binding, and regulation 3. Major metabolic pathways and regulation Respiration, fermentation, and photosynthesis Synthesis and degradation of macromolecules Hormonal control and intracellular messengers 4. Membrane dynamics and cell surfaces Transport, endocytosis, and exocytosis Electrical potentials and transmitter substances Mechanisms of cell recognition, cell junctions, and plasmodesmata Cell wall and extracellular matrix 5. Organelles: structure, function, synthesis, and targeting Nucleus, mitochondria, and plastids Endoplasmic reticulum and ribosomes Golgi apparatus and secretory vesicles Lysosomes, peroxisomes, and vacuoles 1. Genetic foundations Mendelian inheritance Pedigree analysis Prokaryotic genetics (transformation, transduction, and conjugation) Genetic mapping 2. Chromatin and chromosomes Nucleosomes Karyotypes Chromosomal aberrations Polytene chromosomes 3. Genome sequence organization Introns and exons Single-copy and repetitive DNA Transposable elements 4. Genome maintenance DNA replication DNA mutation and repair 5. Gene expression and regulation in prokaryotes and eukaryotes: mechanisms The operon Promoters and enhancers Transcription factors RNA and protein synthesis Processing and modifications of both RNA and protein BIOLOGY TEST PRACTICE BOOK 5 6. Gene expression and regulation: effects Control of normal development Cancer and oncogenes 7. Immunobiology Cellular basis of immunity Antibody diversity and synthesis Antigen-antibody interactions 8. Bacteriophages, animal viruses, and plant viruses Viral genomes, replication, and assembly Virus - host cell interactions 9. Recombinant DNA methodology Restriction endonucleases Blotting and hybridization Restriction fragment length polymorphisms DNA cloning, sequencing, and analysis Polymerase chain reaction II. Organismal Biology (33-34%) 3. Support and movement Support systems (external, internal, and hydrostatic) Movement systems (flagellar, ciliary, and muscular) 4. Integration and control mechanisms Nervous and endocrine systems 5. Behavior (communication, orientation, learning, and instinct) 6. Metabolic rates (temperature, body size, and activity) B. Animal Reproduction and Development (6%) 1. Reproductive structures 2. Meiosis, gametogenesis, and fertilization 3. Early development (e.g., polarity, cleavage, and gastrulation) 4. Developmental processes (e.g., induction, determination, differentiation, morphogenesis, and metamorphosis) 5. External control mechanisms (e.g., photoperiod) C. Plant Structure, Function, and Organization, with Emphasis on Flowering Plants (7%) 1. Organs, tissue systems, and tissues 2. Water transport,...
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This document was uploaded on 03/16/2014 for the course BIO 1434 at UT Arlington.
- Spring '14