Prob Set _5 MCB 102 F 13 ANS KEY

s in mm 0100 0125 0167 0250 0500 1000 1s in

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Unformatted text preview: ] [S] (in mM) 0.100 0.125 0.167 0.250 0.500 1.000 1/[S] (in mM-1) 10 8 6 4 2 1 v0 [in (M s-1) x 107] 0.96 1.12 1.35 1.66 2.22 2.63 1/v0 [in (M-1 s) x 10-7] 1.04 0.89 0.74 0.60 0.45 0.38 Graphing 1/[S] versus 1/v0 from the data in the table above gives the Lineweaver-Burk plot shown on the next page. MCB 102 - Fall 2013 - Prof. J. Thorner The y-intercept is 1/Vmax; so, 1/Vmax = 0.305; therefore, Vmax = 3.28 x 10-7 M s-1 = 0.33 µmole L-1 s-1 The x-intercept is -1/Km; so, -1/Km = -4 mM-1; therefore, Km = 0.25 mM The turnover number (kcat) = Vmax/[ET] and [ET] = 0.01 nM; therefore, kcat = 3.28 x 10-7 M s-1 / 1 x 10-11 M = 3.28 x...
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This note was uploaded on 03/15/2014 for the course MCB 102 taught by Professor Staff during the Fall '08 term at University of California, Berkeley.

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