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Unformatted text preview: ] [S] (in mM)
0.100
0.125
0.167
0.250
0.500
1.000 1/[S] (in mM1)
10
8
6
4
2
1 v0 [in (M s1) x 107]
0.96
1.12
1.35
1.66
2.22
2.63 1/v0 [in (M1 s) x 107]
1.04
0.89
0.74
0.60
0.45
0.38 Graphing 1/[S] versus 1/v0 from the data in the table above gives the LineweaverBurk plot shown on the
next page. MCB 102  Fall 2013  Prof. J. Thorner The yintercept is 1/Vmax; so, 1/Vmax = 0.305; therefore, Vmax = 3.28 x 107 M s1 = 0.33 µmole L1 s1
The xintercept is 1/Km; so, 1/Km = 4 mM1; therefore, Km = 0.25 mM
The turnover number (kcat) = Vmax/[ET] and [ET] = 0.01 nM; therefore,
kcat = 3.28 x 107 M s1 / 1 x 1011 M = 3.28 x...
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This note was uploaded on 03/15/2014 for the course MCB 102 taught by Professor Staff during the Fall '08 term at University of California, Berkeley.
 Fall '08
 Staff

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