{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Prob Set _5 MCB 102 F 13 ANS KEY

s in mm 0100 0125 0167 0250 0500 1000 1s in

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: ] [S] (in mM) 0.100 0.125 0.167 0.250 0.500 1.000 1/[S] (in mM-1) 10 8 6 4 2 1 v0 [in (M s-1) x 107] 0.96 1.12 1.35 1.66 2.22 2.63 1/v0 [in (M-1 s) x 10-7] 1.04 0.89 0.74 0.60 0.45 0.38 Graphing 1/[S] versus 1/v0 from the data in the table above gives the Lineweaver-Burk plot shown on the next page. MCB 102 - Fall 2013 - Prof. J. Thorner The y-intercept is 1/Vmax; so, 1/Vmax = 0.305; therefore, Vmax = 3.28 x 10-7 M s-1 = 0.33 µmole L-1 s-1 The x-intercept is -1/Km; so, -1/Km = -4 mM-1; therefore, Km = 0.25 mM The turnover number (kcat) = Vmax/[ET] and [ET] = 0.01 nM; therefore, kcat = 3.28 x 10-7 M s-1 / 1 x 10-11 M = 3.28 x...
View Full Document

{[ snackBarMessage ]}

Ask a homework question - tutors are online