Prob Set _5 MCB 102 F 13 ANS KEY

001 x 10 4 m 2 x 10 5 m 2 x 10 13 m2 s 1 1201 x 10 4

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Unformatted text preview: 001 x 10-4 M + 2 x 10-5 M) = 2 x 10-13 M2 s-1 / 1.201 x 10-4 M = 1.67 x 10-9 M s-1 = 1.67 nmole L-1 s-1 Question 3: You are studying another enzyme and you conduct an analysis of the effect of substrate concentration on reaction velocity. At each substrate concentration, you initiate the reaction by adding the enzyme at the same final concentration (0.01 nM), incubate for 5 min, and then measure the amount of product formed. You obtain the initial velocity data shown in the table below. On the basis of these data, calculate Km, Vmax, kcat and the specificity constant. [Hint: You should analyze these data graphically....
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