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Unformatted text preview: 104 s-1; or, in other words,
32,800 molecules of S converted to product per second per enzyme molecule
The specificity constant is defined as kcat/Km = 3.38 x 104 s-1 / 2.5 x 10-4 M = 1.35 x 108 M-1 s-1
Question 4: As a summer job, you work in a lab doing research on developing alternative sources of energy, which is
trying to exploit the metabolism of a photosynthetic green alga. As in plants (and some bacteria, like E. coli, and
certain yeasts, like S. cerevisiae), this microbe has a pathway (the glyoxylate cycle) by which it converts acetic acid
into TCA cycle intermediates. Your task is to study one of the critical enzymes of this cycle, isocitrate lyase (which
splits isocitrate into succinate and glyoxylate). You...
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This note was uploaded on 03/15/2014 for the course MCB 102 taught by Professor Staff during the Fall '08 term at University of California, Berkeley.
- Fall '08