Prob Set _5 MCB 102 F 13 ANS KEY

28 x 104 s 1 or in other words 32800 molecules of s

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Unformatted text preview: 104 s-1; or, in other words, 32,800 molecules of S converted to product per second per enzyme molecule The specificity constant is defined as kcat/Km = 3.38 x 104 s-1 / 2.5 x 10-4 M = 1.35 x 108 M-1 s-1 Question 4: As a summer job, you work in a lab doing research on developing alternative sources of energy, which is trying to exploit the metabolism of a photosynthetic green alga. As in plants (and some bacteria, like E. coli, and certain yeasts, like S. cerevisiae), this microbe has a pathway (the glyoxylate cycle) by which it converts acetic acid into TCA cycle intermediates. Your task is to study one of the critical enzymes of this cycle, isocitrate lyase (which splits isocitrate into succinate and glyoxylate). You...
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