Prob Set _5 MCB 102 F 13 ANS KEY

Prob Set _5 MCB 102 F 13 ANS KEY - MCB 102 Fall 2013 Prof J...

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MCB 102 - Fall 2013 - Prof. J. Thorner Problem Set #5 Question 1: When developing and applying an assay to measure the activity of a particular enzyme, does it matter whether you assess the rate of appearance of the product or the rate of disappearance of the substrate? Theoretically , it does not matter whether it is the appearance of the products(s) or the disappearance of the substrate(s) that is monitored. However, there are some important practical considerations. Partly, the decision is based on which chemical substance is easiest to quantify analytically. However, another very important consideration is that it is often easiest to determine most accurately the amount of product formed because product formation proceeds from zero to some quantity. In contrast, it is harder to determine the decrease in reactants with high accuracy because you may be required to measure what could be just a small decrease in a relatively large starting value. So, all things being equal, most enzymologists measure the rate of product formation, if at all possible. Question 2: You have collected experimental data that show that an enzyme you are studying follows the kinetic scheme below. [Hint: Recall the assumptions that underlie the steady-state approximation and are the basis of the Michaelis-Menten treatment of enzyme-catalyzed reactions.] k 1 k 3 E + S E-S E + P k 2 k 4 where k 1 = 10 9 M -1 s -1 ; k 2
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