Prob Set _5 MCB 102 F 13 ANS KEY

S in mm 00318 00464 00593 01185 02222 1 1s mm

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Unformatted text preview: (in mM) 0.0318 0.0464 0.0593 0.1185 0.2222 -1 1/[S] (mM ) 31.45 21.55 16.86 8.44 4.50 µmoles glyoxylate per 10 min, -OAA 0.0420 0.0583 0.0700 0.0955 0.1167 1/V0, - Inhib. 23.81 17.15 14.29 10.47 8.57 µmoles glyoxylate per 10 min, +OAA 0.0040 0.0055 0.0075 0.0131 0.0233 1/V0, + Inhib. 250 182 133 76.3 42.9 MCB 102 - Fall 2013 - Prof. J. Thorner As in the answer to Ques. #3, it is simplest to graph the data shown in the table as a 1/[S] versus 1/v0 plot, which yields the results shown on the next page. Both lines intersect the y-axis at the same point, namely approx. 7.1; therefore, Vmax = 1 / 7.1 = 0.14 µM pe...
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This note was uploaded on 03/15/2014 for the course MCB 102 taught by Professor Staff during the Fall '08 term at Berkeley.

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