Prob Set _5 MCB 102 F 13 ANS KEY

You add a partially purified preparation of the

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Unformatted text preview: add a partially purified preparation of the enzyme to a reaction buffer that contains different initial substrate concentrations, in the presence and absence of another TCA cycle intermediate, oxaloacetate (OAA), that you suspect may be an inhibitor of your algal isocitrate lysase. After incubation for 10 min, you terminate the reaction by heating each tube to denature the enzyme, and measure the amount of glyoxylate produced using a spectrophotometric method. You obtain the initial velocity data shown in the table below. What are Km and Vmax for your enzyme? Is OAA an inhibitor? If not, why not? If so, what kind of inhibitor? [S]...
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This note was uploaded on 03/15/2014 for the course MCB 102 taught by Professor Staff during the Fall '08 term at University of California, Berkeley.

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