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Unformatted text preview: add a partially purified preparation of the enzyme to a reaction
buffer that contains different initial substrate concentrations, in the presence and absence of another TCA cycle
intermediate, oxaloacetate (OAA), that you suspect may be an inhibitor of your algal isocitrate lysase. After
incubation for 10 min, you terminate the reaction by heating each tube to denature the enzyme, and measure the
amount of glyoxylate produced using a spectrophotometric method. You obtain the initial velocity data shown in the
table below. What are Km and Vmax for your enzyme? Is OAA an inhibitor? If not, why not? If so, what kind of
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This note was uploaded on 03/15/2014 for the course MCB 102 taught by Professor Staff during the Fall '08 term at University of California, Berkeley.
- Fall '08