100L_DataSheet_2-12_StandardPlateCount.docx - MIMG100L Name...

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MIMG100L Name:_____________________________________ Date______________ Lab Section:____ UID:_________________________ I was present and performed this exercise (initial) _______________ D ATA S HEET 2-12: S TANDARD P LATE C OUNT PRE-LAB: Describe the Purpose or Objective of this exercise: Draw (by hand or using the digital tool of your choice) a simple flow chart depicting the order of work (steps) for this exercise. 1
D ATA S HEET 2-12: STANDARD PLATE COUNT EXPERIMENT PERFORMED: Escherichia coli ( E. coli ) was serially diluted as described in Figure 2.45 (p.120) of the Lab Manual. Since we predicted that the dilution series outlined in the manual may not dilute the E. coli enough for us to see single colonies, we decided to dilute the E. coli stock solution out to tube 6 and then plated out 5 plates instead of 4. The plates were incubated overnight at 35°C. RESULTS: O BSERVATIONS AND I NTERPRETATIONS 2 C A D E B
D ATA S HEET 2-12: STANDARD PLATE COUNT 1. Enter the number of colonies on each countable plate. Plates with >300 colonies, write “TNTC” (too numerous to count).

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