6-Control_of_Microorganisms_Feb7

Minimum bacteriocidal concentration mbc is the lowest

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: ied as: •  Bacteriostatic: inhibit growth of microorganism. •  Bacteriocidal: kill microorganism. •  Bacteriolytic: kill microorganism by inducing lysis. Brock Biology of Microorganisms, 13th Edition. Measuring an4microbial ac4vity •  Minimum inhibitory concentration (MIC) is the smallest amount of an agent needed to inhibit growth of a microorganism Concentration (10-fold serial dilution) •  Varies with the organism used, inoculum size, temp, pH, etc. •  Minimum lethal concentration (MLC) is the lowest concentration of an agent that kills a test organism. •  Minimum bacteriocidal concentration (MBC) is the lowest concentration of an agent that kills a test bacterium. Viable counts à༎ MLC, MBC •  Decimal reduction time, Decimal reduction concentration. “Kills 99.9% of germs in 30s”. Brock Biology of Microorganisms, 13th Edition. Survival fraction (log scale) D10 Decimal reduction concentration Concentration of active compound Measuring an4microbial ac4vity •  Disc diffusion assay •  Antimicrobial agent added to filter paper disc. •  MIC is reached at some distance from the disc. •  Zone of inhibition: Area of no growth around the d...
View Full Document

Ask a homework question - tutors are online