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Unformatted text preview: , New York, United States of America, 4 Medicine, Albert Einstein College of Medicine, Albert Einstein College of Medicine, Bronx, New York,
United States of America Background. Melanin pigments are ubiquitous in nature. Melanized microorganisms are often the dominating species in
certain extreme environments, such as soils contaminated with radionuclides, suggesting that the presence of melanin is
beneficial in their life cycle. We hypothesized that ionizing radiation could change the electronic properties of melanin and
might enhance the growth of melanized microorganisms. Methodology/Principal Findings. Ionizing irradiation changed the
electron spin resonance (ESR) signal of melanin, consistent with changes in electronic structure. Irradiated melanin manifested
a 4-fold increase in its capacity to reduce NADH relative to non-irradiated melanin. HPLC analysis of melanin from fungi grown
on different substrates revealed chemical complexity, dependence of melanin composition on the growth substrate and
possible influence of melanin composition on its interaction with ionizing radiation. XTT/MTT assays showed increased
metabolic activity of melanized C. neoformans cells relative to non-melanized cells, and exposur...
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This note was uploaded on 03/20/2014 for the course PSYC 101 taught by Professor Catone during the Spring '07 term at CSU Fullerton.
- Spring '07