The nonproducing strains cannot be differentiated by

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Unformatted text preview: their increasing global occurrence, pose a serious threat to public health, domestic animals, and livestock. In Missisquoi Bay, Lake Champlain, public health advisories have been issued from 2001 to 2009, and local microcystin concentrations found in the lake water regularly exceeded the Canadian drinking water guideline of 1.5 g liter 1. A quantitative PCR (Q-PCR) approach was developed for the detection of blooms formed by microcystin-producing cyanobacteria. Primers were designed for the -ketoacyl synthase (mcyDKS) and the first dehydratase domain (mcyDDH) of the mcyD gene, involved in microcystin synthesis. The Q-PCR method was used to track the toxigenic cyanobacteria in Missisquoi Bay during the summers of 2006 and 2007. Two toxic bloom events were detected in 2006: more than 6.5 104 copies of the mcyDKS gene ml 1 were detected in August, and an average of 4.0 104 copies ml 1 were detected in September, when microcystin concentrations were more than 4 g liter 1 and approximately 2 g liter 1, respectively. Gene copy numbers and total microcystin concentrations (determined by enzyme-linked immunoso...
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