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Unformatted text preview: their increasing global occurrence, pose a serious threat to public
health, domestic animals, and livestock. In Missisquoi Bay, Lake Champlain, public health advisories have
been issued from 2001 to 2009, and local microcystin concentrations found in the lake water regularly exceeded
the Canadian drinking water guideline of 1.5 g liter 1. A quantitative PCR (Q-PCR) approach was developed
for the detection of blooms formed by microcystin-producing cyanobacteria. Primers were designed for the
-ketoacyl synthase (mcyDKS) and the ﬁrst dehydratase domain (mcyDDH) of the mcyD gene, involved in
microcystin synthesis. The Q-PCR method was used to track the toxigenic cyanobacteria in Missisquoi Bay
during the summers of 2006 and 2007. Two toxic bloom events were detected in 2006: more than 6.5
copies of the mcyDKS gene ml 1 were detected in August, and an average of 4.0 104 copies ml 1 were detected
in September, when microcystin concentrations were more than 4 g liter 1 and approximately 2 g liter 1,
respectively. Gene copy numbers and total microcystin concentrations (determined by enzyme-linked immunoso...
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This note was uploaded on 03/20/2014 for the course PSYC 101 taught by Professor Catone during the Spring '07 term at CSU Fullerton.
- Spring '07