Invitrogen - Low mass DNA Ladder

Storage buffer 10 mm tris hc1 ph 75 1 mm edta storage

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Unformatted text preview: respectively. Storage Buffer 10 mM Tris-HC1 (pH 7.5), 1 mM EDTA Storage Buffer 10 mM Tris-HC1 (pH 7.5), 1 mM EDTA Preparing the Ladder with Loading Dye Mix four volumes of Low DNA Mass Ladder with one volume of gel loading buffer containing dye (e.g., 4 µl ladder with 1 µl dye). Then load the ladder/dye mixture on the gel. Preparing the Ladder with Loading Dye Mix four volumes of Low DNA Mass Ladder with one volume of gel loading buffer containing dye (e.g., 4 µl ladder with 1 µl dye). Then load the ladder/dye mixture on the gel. The table on page 2 shows the appropriate volume of ladder to use to estimate the mass of unknown DNA samples. For reliable comparison of band intensities, the loading volume of the ladder should be the same as the volume of the experimental sample. Smaller volumes routinely give sharper bands. Do not heat the ladder before loading. The table on page 2 shows the appropriate volume of ladder to use to estimate the mass of unknown DNA samples. For reliable comparison of band intensities, the loading volume of the ladder should be the same as the volume of the experimental sample. Smaller volumes routinely give sharper bands. Do not heat the ladder before loading. Note: The closer the size of the sample band relative to the band of comparable intensity in the Low DNA Mass Ladder, the more accurate the mass estimation will be. Note: The closer the size of the sample band relative to the band of comparable intensity in the Low DNA Mass Ladder, the more accurate the mass estimation will be. Quality Control Agarose gel analysis shows that all bands (2000, 1200, 800, 400, 200, 100 bp) are clearly distinguishable. Sharpness and relative intensity of the bands and the absence of background are evaluated relative to a control lot by agarose gel analysis. Quality Control Agarose gel analysis shows that all bands (2000, 1200, 800, 400, 200, 100 bp) are clearly dist...
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