Unformatted text preview: ugh the system at different rates.
These differential rates of migration as the mixture
moves over adsorptive materials provide separation.
Repeated sorption/desorption acts that take place during
the movement of the sample over the stationary bed
determine the rates.
The smaller the affinity a molecule has for the stationary
phase, the shorter the time spent in a column. Chromatography is a very special separation process for
a multitude of reasons
It can separate complex mixtures with great precision.
Even very similar components, such as proteins that
may only vary by a single amino acid, can be separated
Chromatography can purify basically any soluble or
volatile substance if the right adsorbent material, carrier
fluid, and operating conditions are employed.
Chromatography can be used to separate delicate
products since the conditions under which it is performed
are not typically severe.
Chromatography is quite well suited to a variety of uses
in the field of biotechnology, such as separating mixtures
of There is an equilibrium of solute molecules
being adsorbed to the solid surface and
desorbed and dissolved in the mobile solvent
Those molecules that are most soluble in the
solvent move faster while those that a less
soluble spend more time adsorbed to the
stationary phase and move slowly
Therefore mixtures are separated into bands –
separated by polar functional groups
separated Partition (liquid-liquid) chromatography
– Separation of solute is base upon relative
solubility in an organic (non-polar) sol...
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This document was uploaded on 03/22/2014.
- Spring '14