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Clinical Chemistry 5 Chromatography and Electrophoresis

The the ratio of the concentration of the solute in

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Unformatted text preview: vent and the aqueous (polar) solvent. the – The ratio of the concentration of the solute in The the two liquid is known as the partition coefficient coefficient Partition Chromatography Partition Separation of solute is based upon relative Separation solubility in an organic (non-polar) solvent and an aqueous (polar) solvent an The ratio of the concentration of the solute in the The two liquids is known as the partition coefficient two Compounds that are most soluble in the mobile Compounds phase move through the column faster than do compounds with a high partition coefficient which stay in the stationary phase Steric Exclusion Steric A variation of liquid-solid chromatography is variation used to separate solute molecules on the basis of size and shape. of The column is packed with a porous material. A sample containing different-sized molecules sample moves down the column dissolved in the mobile solvent. solvent. Small molecules enter the pores in the packing Small and are momentarily trapped. and Large molecules are excluded from the small Large pores and move quickly. Intermediate molecules are partially trapped are Ion Exchange Ion Solute mixtures are separated by virtue of Solute ionic charge ionic The stationary phase is the resin The consisting of large polymers with charged functional groups functional cation exchange – for example H+ (from cation acid) can exchange with Na+ ...
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