Clinical chemistry 6 Electrophoresis

Afterstainingtheseparatedmacromoleculesin

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Unformatted text preview: forced to move through the pores when the electrical current is applied. Their rate of migration through the electric field depends on the strength of the field, size and shape of the molecules, relative hydrophobicity of the samples, and on the ionic strength and temperature of the buffer in which the molecules are moving. After staining, the separated macromolecules in each lane can be seen in a series of bands spread from one end of the gel to the other. To completely understand the separation of charged particles in gel electrophoresis, it is important to look at some simple equations relating to electrophoresis. When a potential difference (voltage) is applied across the electrodes, it generates a potential gradient (E), which is the applied voltage (V) divided by the distance (d) between the electrodes. E = V / d When the potential gradient E is applied, the force on a molecule bearing a charge of q coulombs is Eq Newton's. F = Eq It is this force that drives a charged molecule towards an electrode. There is also a frictional resistance that slows down t...
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This document was uploaded on 03/22/2014.

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