Clinical chemistry 6 Electrophoresis

Anincreasedrateofdiffusionofsampleandbufferionsdueto

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Unformatted text preview: r the f, the slower it will migrate; – Depends on the size and shape of the migrating molecules, and also the viscosity of the medium through which the The current in the solution between the electrodes is conducted mainly by the buffer ions with a small proportion being conducted by the sample ions. Ohm's law expresses the relationship between current (I), voltage (V), and resistance (R): R = V / I This equation demonstrates that it is possible to accelerate an electrophoretic separation by increasing the applied voltage, which would result in a corresponding increase in the current flow. The distance migrated will be proportional to both current and time. – – – – However, increasing the voltage would ignore one of the major problems for most forms of electrophoresis, namely the generation of heat. An increased rate of diffusion of sample and buffer ions due to heat leads to broadening of the separated samples. The formation of convection currents, which leads to mixing of separated samples. Thermal instability of samples that are rather sensitive to heat. This may include denaturation of proteins or loss...
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