Clinical chemistry 6 Electrophoresis

Inthiswaythepercentageofpolyacrylamidecanbecontrolled

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Unformatted text preview: of activity of enzymes. A decrease of buffer viscosity, and hence a reduction in the resistance of the medium. The Support Medium, Gel The Support Medium, Gel There are two basic types of materials used to make gels: agarose and polyacrylamide. – Agarose, used for RNA and DNA (we will learn it in Lab #10) – Polyacrylamide, used for small proteins The gel pore size can be controlled by using different percentage of polyacrylamide; High degree of resolution; Neurotoxin when in the depolarized state (liquid form). Polyacrylamide Polyacrylamide The polyacrylamide gel electrophoresis (PAGE) technique was introduced by Raymond and Weintraub (1959). Polyacrylamide is the same material that is used for skin electrodes and in soft contact lenses. Polyacrylamide gel may be prepared so as to provide a wide variety of electrophoretic conditions. – The pore size for the gel may be varied to produce different molecular sieving effects for separating proteins of different sizes. – In this way, the percentage of polyacrylamide can be controlled in a given gel. Polyacrylamide gels can be cast in a single percentage or with varying gradients. – Gradient gels provide...
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This document was uploaded on 03/22/2014.

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