{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Clinical chemistry 6 Electrophoresis

Inthiswaythepercentageofpolyacrylamidecanbecontrolled

Info icon This preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: of activity of enzymes. A decrease of buffer viscosity, and hence a reduction in the resistance of the medium. The Support Medium, Gel The Support Medium, Gel There are two basic types of materials used to make gels: agarose and polyacrylamide. – Agarose, used for RNA and DNA (we will learn it in Lab #10) – Polyacrylamide, used for small proteins The gel pore size can be controlled by using different percentage of polyacrylamide; High degree of resolution; Neurotoxin when in the depolarized state (liquid form). Polyacrylamide Polyacrylamide The polyacrylamide gel electrophoresis (PAGE) technique was introduced by Raymond and Weintraub (1959). Polyacrylamide is the same material that is used for skin electrodes and in soft contact lenses. Polyacrylamide gel may be prepared so as to provide a wide variety of electrophoretic conditions. – The pore size for the gel may be varied to produce different molecular sieving effects for separating proteins of different sizes. – In this way, the percentage of polyacrylamide can be controlled in a given gel. Polyacrylamide gels can be cast in a single percentage or with varying gradients. – Gradient gels provide...
View Full Document

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern